干扰素人类基因在酵母中的表达。
Expression of a human gene for interferon in yeast.
作者信息
Hitzeman R A, Hagie F E, Levine H L, Goeddel D V, Ammerer G, Hall B D
出版信息
Nature. 1981 Oct 29;293(5835):717-22. doi: 10.1038/293717a0.
DOI:10.1038/293717a0
PMID:6169997
Abstract
A DNA sequence coding for mature human leukocyte interferon D (LeIF-D) was linked with DNA fragments of the 5'-flanking sequences of the Saccharomyces cerevisiae (yeast) alcohol dehydrogenase I gene in a plasmid capable of autonomous replication and selection in both yeast and Escherichia coli. Yeast cells transformed by these plasmids synthesize up to 1 x 10(6) molecules of biologically active LeIF-D per cell.
摘要
编码成熟人白细胞干扰素D（LeIF-D）的DNA序列与酿酒酵母（酵母）乙醇脱氢酶I基因5'侧翼序列的DNA片段连接在一个能够在酵母和大肠杆菌中自主复制和筛选的质粒中。用这些质粒转化的酵母细胞每个细胞可合成多达1×10⁶个生物活性LeIF-D分子。
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