Ovsiannikova N V, Karmysheva V Ia
Vopr Virusol. 1980 Jan-Feb(1):72-6.
Virus-specified immune cytolysis has been shown in mouse cell cultures infected with vesicular stomatitis virus under the action of specific virus antibody and active complement. The exogenous mouse interferon failed to enhance the cellular plasmalemma permeability or to inhibit cellular adsorption of infectious and inactivated virus as well as the lysis of the cells in the presence of the antiviral serum and complement. The interferon localization was studied by the method of fluorescent antibodies. In vital preparations the exogenous species-specific interferon was revealed on the surface of most cells in the treated cultures; in the fixed preparations of the same cultures the interferon was found only in the form of small lumps in the cytoplasm of few cells. A decrease in immune cytolysis was observed in the cultures treated with the interferon 20-24 hours before the infection, that can be accounted for by the inhibitory action of the interferon on the viral replication and the reduction of the number of the virus-inducing antigens in the cellular plasmalemma.
在特定病毒抗体和活性补体的作用下，感染水疱性口炎病毒的小鼠细胞培养物中已显示出病毒特异性免疫细胞溶解作用。外源性小鼠干扰素未能增强细胞浆膜通透性，也未能抑制感染性和灭活病毒的细胞吸附以及在抗病毒血清和补体存在下细胞的裂解。采用荧光抗体法研究了干扰素的定位。在活体制备物中，在处理过的培养物中的大多数细胞表面发现了外源性种属特异性干扰素；在相同培养物的固定制剂中，仅在少数细胞的细胞质中发现干扰素呈小团块形式。在感染前20 - 24小时用干扰素处理的培养物中观察到免疫细胞溶解作用降低，这可归因于干扰素对病毒复制的抑制作用以及细胞浆膜中病毒诱导抗原数量的减少。
