Zółtowska A, Winnicka U, Moszkowska G
Arch Immunol Ther Exp (Warsz). 1980;28(1):61-5.
After inducing amyloidosis in hamsters by hyperimmunization with Di-Te-Per vaccine, their serum proteins were separated by electrophoresis. Results showed that the level of alpha 1 globulin increased and that of albumin decreased after 6-8 weeks of the hyperimmunization. Gamma-globulins increased by the 10th week of the study, then their level markedly decreased. Electrophoretic separation of the serum proteins refrigerated for 24 h at 4 degrees C showed the presence of a steadily increasing protein fraction induced by the prolonged immunization, which migrated in the prealbumin band. The fraction was observed in all phases of the experiments. This fraction was stained using selective methods for lipids. New bands appearing during electrophoretic separation of hamster serum proteins in amyloidosis may represent monomeric or polimeric forms of amyloid protein.
用二碲过氧化物疫苗对仓鼠进行超免疫诱导淀粉样变性后，通过电泳分离它们的血清蛋白。结果显示，超免疫6 - 8周后，α1球蛋白水平升高，白蛋白水平降低。到研究第10周时γ球蛋白增加，随后其水平显著下降。在4℃冷藏24小时的血清蛋白的电泳分离显示，长期免疫诱导产生了一个不断增加的蛋白组分，它在前白蛋白区带迁移。在实验的所有阶段都观察到了该组分。使用脂质选择性方法对该组分进行染色。淀粉样变性仓鼠血清蛋白电泳分离过程中出现的新条带可能代表淀粉样蛋白的单体或多聚体形式。
