Zahorska R, Korbecki M, Barciszewski J
Serum and Vaccine Research Laboratory, Warsaw, Poland.
Arch Immunol Ther Exp (Warsz). 1995;43(1):43-6.
In the presence and absence of methionine initiator tRNA (tRNA(iMet)) theophylline applied for 1 h starting 6 h after induction increased significantly (2-fold) interferon (IFN) yield. Theophylline did not change the kinetics of IFN production in the presence of tRNA(iMet). The shut-off stage was present and the production was prolonged to 24 h after IFN induction. In comparison with the values obtained in untreated cells, poly I:C plus DEAE dextran (inducing complex) caused an about 2-2.5 fold increase of cyclic adenosine 3'5'-monophosphate (cAMP) level. The similar results were observed in the cells treated with inducing complex plus tRNA(iMet). About 2-fold higher cAMP level occurred in the cells under the influence of the inducing complex plus theophylline in comparison to the level obtained without theophylline. The addition of tRNA(iMet) did not influence the above presented data.
在有和没有甲硫氨酸起始tRNA（tRNA(iMet)）的情况下，诱导后6小时开始应用1小时的茶碱可使干扰素（IFN）产量显著增加（2倍）。在存在tRNA(iMet)的情况下，茶碱不会改变IFN产生的动力学。存在关闭阶段，并且在IFN诱导后产生延长至24小时。与未处理细胞中获得的值相比，聚肌苷酸-聚胞苷酸（poly I:C）加二乙氨基乙基葡聚糖（DEAE葡聚糖）（诱导复合物）使环磷酸腺苷（cAMP）水平增加约2-2.5倍。在用诱导复合物加tRNA(iMet)处理的细胞中观察到类似结果。与未用茶碱处理获得的水平相比，在诱导复合物加茶碱影响下的细胞中cAMP水平高出约2倍。添加tRNA(iMet)不影响上述数据。
