单周期试验中干扰素对细胞溶解性T淋巴细胞的作用
Interferon effect on cytolytic T lymphocytes in a single cycle assay.
作者信息
Bergeret M, Fouchard M, Gregoire A, Chany C, Zagury D
出版信息
Immunology. 1983 Jan;48(1):101-6.
In this study we analyse cell-mediated cytotoxicity by the use of 51chromium release and the killer cell enumeration assay. The latter enabled us to estimate cell-mediated cytotoxicity in a single cycle, which confirms the protective effect of interferon (IFN) after target cell treatment. This anti-cytolytic resistance is detected best by microassociation when effector and target cells are separately treated with IFN and associated thereafter. We suggest that, in inflammatory areas, enhanced cytotoxic activity of the IFN-treated effector cells is only operative before the establishment of protection in the targets, which is somewhat slower and appears in about 18 hr. Resistance of the targets could be at least partly attributed to the incapacity of effector cells to bind to them.
在本研究中，我们通过使用51铬释放法和杀伤细胞计数测定法来分析细胞介导的细胞毒性。后者使我们能够在单个周期内估计细胞介导的细胞毒性，这证实了靶细胞处理后干扰素（IFN）的保护作用。当效应细胞和靶细胞分别用IFN处理并随后联合时，通过微联合能最好地检测到这种抗细胞溶解抗性。我们认为，在炎症区域，经IFN处理的效应细胞增强的细胞毒性活性仅在靶细胞建立保护之前起作用，这种保护作用建立得稍慢，约在18小时后出现。靶细胞的抗性至少部分可归因于效应细胞无法与它们结合。
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