Kapikian A Z, Cline W L, Kim H W, Kalica A R, Wyatt R G, Vankirk D H, Chanock R M, James H D, Vaughn A L
Proc Soc Exp Biol Med. 1976 Sep;152(4):535-9. doi: 10.3181/00379727-152-39434.
The human reovirus-like (HRVL) agent, Nebraska calf diarrhea virus (NCDV), epizootic diarrhea of infant mice (EDIM) virus, simian agent (SA)-11, and the "O" (offal) agent were found to be similar, if not identical, in reciprocal complement fixation (CF) tests employing hyperimmune animal sera. In addition, in CF tests with paired sera from 35 diarrhea patients who shed the HRVL agent, 74% developed serologic evidence of infection with the HRVL antigen, 43% with NCDV, 51% with EDIM virus, 57% with SA-11, and 71% with the "O" agent. Thus, in addition to the NCDV, which had previously been described as a suitable substitute CF antigen for the HRVL agent, the SA-11, "O", and EDIM viruses may also be utilized as substitute antigens for the HRVL agent. However, the "O" agent appears to be the most efficient of the four substitute CF antigens and thus should be used preferentially when the HRVL agent is not available. The "O" agent was about as efficient as the HRVL agent and significantly more efficient than the NCDV for detecting seroresponses. The greatest efficiency for detecting infection with the HRVL agent resulted when sera were tested with both the HRVL and "O" agents as 31 (89%) of the patients developed serologic evidence of infection with one or both antigens. The finding of additional substitute CF antigens for the HRVL agent may have implications in the immunoprophylaxis against human disease.
在使用超免疫动物血清进行的双向补体结合（CF）试验中，发现人类呼肠孤病毒样（HRVL）病原体、内布拉斯加小牛腹泻病毒（NCDV）、婴儿小鼠流行性腹泻（EDIM）病毒、猴病毒（SA）-11和“O”（内脏）病原体即便不完全相同，也极为相似。此外，在对35名排出HRVL病原体的腹泻患者的配对血清进行的CF试验中，74%的患者产生了感染HRVL抗原的血清学证据，43%感染NCDV，51%感染EDIM病毒，57%感染SA-11，71%感染“O”病原体。因此，除了先前被描述为HRVL病原体合适替代CF抗原的NCDV外，SA-11、“O”和EDIM病毒也可作为HRVL病原体的替代抗原。然而，“O”病原体似乎是四种替代CF抗原中最有效的，因此在无法获得HRVL病原体时应优先使用。“O”病原体在检测血清反应方面与HRVL病原体相当，且比NCDV显著更有效。当同时用HRVL和“O”病原体检测血清时，检测HRVL病原体感染的效率最高，因为31名（89%）患者产生了感染一种或两种抗原的血清学证据。发现HRVL病原体的额外替代CF抗原可能对人类疾病的免疫预防具有重要意义。
