呼肠孤病毒样肠炎病原体的血清学研究。
Serological studies with reovirus-like enteritis agent.
作者信息
Blacklow N R, Echeverria P, Smith D H
出版信息
Infect Immun. 1976 Jun;13(6):1563-6. doi: 10.1128/iai.13.6.1563-1566.1976.
Abstract
The age distribution of antibody to the human reovirus-like enteritis agent, and to the antigenically related Nebraska calf diarrhea virus (NCDV) was studied in serum specimens obtained from 592 children hospitalized in Boston. Sera were examined for complement-fixation (CF) antibody to the human agent, and for CF and indirect immunofluorescence-staining antibodies to NCDV. The curve of antibody frequency was similar in each of the three assays, showing a steep rise in the 6- to 18-month-old age groups; these results indicate the early acquisition of antibody to the reovirus-like enteritis agent. The majority of children and young adults possessed CF- and immunofluorescence-stainable antibodies. There was a significant association between antibody prevalence data obtained with the human CF antigen and with the two NCDV antigens; this association was closest between the human and NCDV CF antigens.
摘要
对从波士顿住院的592名儿童采集的血清标本，研究了针对人类呼肠孤病毒样肠炎病原体以及抗原相关的内布拉斯加小牛腹泻病毒（NCDV）抗体的年龄分布情况。检测血清中针对人类病原体的补体结合（CF）抗体，以及针对NCDV的CF和间接免疫荧光染色抗体。在这三种检测中，抗体频率曲线相似，在6至18个月龄组中呈急剧上升；这些结果表明早期获得了针对呼肠孤病毒样肠炎病原体的抗体。大多数儿童和年轻人拥有CF和免疫荧光可染色抗体。用人CF抗原和两种NCDV抗原获得的抗体流行率数据之间存在显著关联；这种关联在人CF抗原和NCDV CF抗原之间最为密切。
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Detection and localization of viruses in human fetal intestinal organ cultures by immunofluorescence.通过免疫荧光法检测和定位人胎儿肠道器官培养物中的病毒。
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