Borgström A, Ohlsson K
Hoppe Seylers Z Physiol Chem. 1980 May;361(5):625-31. doi: 10.1515/bchm2.1980.361.1.625.
Radioimmunoassays for anionic and cationic dog trypsins are described. Characterization of the immunoreactivities in sera from fasting dogs demonstrated the presence of the two proenzymes only. Fasting sera from 10 dogs contained anionic and cationic trypsinogen in concentrations between 17-110 micrograms/l and 7-19 micrograms/l, respectively. Induction of experimental pancreatitis in dogs was accompanied by a large increase of immunoreactive anionic and cationic trypsins in the circulation. During the progress of the pancreatitis, immunoreactive trypsin with larger molecular weight than trypsinogen appeared. This high molecular weight immunoreactive trypsin was not seen in serum after intravenous injection of pancreatic juice in dogs. The high molecular weight immunoreactive trypsin probably consists of trypsin in complex with protease inhibitors. In vitro studies showed that the immunoreactivity of trypsin decreased considerably after binding to alpha 1-antitrypsin or alpha-macroglobulins.
本文描述了针对阴离子型和阳离子型犬胰蛋白酶的放射免疫分析方法。对空腹犬血清中免疫反应性的表征显示，仅存在两种酶原。10只犬的空腹血清中，阴离子型和阳离子型胰蛋白酶原的浓度分别在17 - 110微克/升和7 - 19微克/升之间。犬实验性胰腺炎的诱导伴随着循环中免疫反应性阴离子型和阳离子型胰蛋白酶的大幅增加。在胰腺炎进展过程中，出现了分子量比胰蛋白酶原更大的免疫反应性胰蛋白酶。在犬静脉注射胰液后，血清中未观察到这种高分子量的免疫反应性胰蛋白酶。这种高分子量的免疫反应性胰蛋白酶可能由与蛋白酶抑制剂结合的胰蛋白酶组成。体外研究表明，胰蛋白酶与α1 -抗胰蛋白酶或α -巨球蛋白结合后，其免疫反应性显著降低。
