Groome N P
J Clin Chem Clin Biochem. 1980 Jun;18(6):345-9. doi: 10.1515/cclm.1980.18.6.345.
Most of the currently used enzyme immunological assays employ horse radish peroxidase as marker enzyme. A comparison is presented of ABTS (2,2'-azino-di-(3-ethyl benzthiazoline-6-sulphonic acid) and the Trinder reagent as chromogens for the detection of small amounts of solid-phase peroxidase. The formation of chromophore using the Trinder reagent under the conditions described by Gallati (J. Clin. Chem. Clin. Biochem. (1977) 15, 699-703) reaches a plateau after 3-4 h due to H2O2 induced inactivation of the enzyme. In contrast, with suitable temperature and concentrations of H2O2 and ABTS, chromophore production continues in this system for at least 20 h. In an Enzyme Linked Immunoadsorbent Assay for antibodies to myelin basic protein the use of the ABTS/H2O2 substrate system described here gives an assay 14 times more sensitive than the maximum possible with Trinder reagent.
目前大多数酶免疫分析方法都使用辣根过氧化物酶作为标记酶。本文比较了ABTS（2,2'-叠氮-二-（3-乙基苯并噻唑啉-6-磺酸））和Trinder试剂作为检测少量固相过氧化物酶的显色剂。在Gallati（《临床化学与临床生物化学杂志》（1977年）15卷，699 - 703页）所述条件下，使用Trinder试剂形成发色团时，由于H2O2诱导酶失活，3 - 4小时后达到平台期。相比之下，在合适的温度、H2O2和ABTS浓度下，该体系中发色团的产生至少持续20小时。在检测抗髓鞘碱性蛋白抗体的酶联免疫吸附测定中，使用本文所述的ABTS/H2O2底物系统，其检测灵敏度比使用Trinder试剂时的最高可能灵敏度高14倍。
