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在ABTS过氧化物酶培养基上检测微生物产生的过氧化氢。

Detection of hydrogen peroxide produced by microorganisms on an ABTS peroxidase medium.

作者信息

Müller H E

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Apr;259(2):151-4. doi: 10.1016/s0176-6724(85)80045-6.

DOI:10.1016/s0176-6724(85)80045-6
PMID:4013558
Abstract

Previously published methods to detect the production of hydrogen peroxide by microorganisms have been of limited usefulness for routine diagnosis because the sensitivity of some of these procedures does not allow the isolation of single H2O2-producing colonies. The new medium containing 2,2'-azino-di (3-ethyl benzthiazoline-6-sulphonic acid) and horseradish peroxidase is more sensitive and faster than established methods.

摘要

先前已发表的检测微生物产生过氧化氢的方法对于常规诊断用处有限,因为其中一些方法的灵敏度不允许分离出单个产生H2O2的菌落。含有2,2'-叠氮基-二(3-乙基苯并噻唑啉-6-磺酸)和辣根过氧化物酶的新培养基比现有方法更灵敏、更快速。

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