Langer R, Conn H, Vacanti J, Haudenschild C, Folkman J
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4331-5. doi: 10.1073/pnas.77.7.4331.
Angiogenesis and tumor growth were inhibited in two different animal models by regional infusion of a partially purified cartilage extract. In rabbits bearing corneal implants of V2 carcinoma and receiving the inhibitor, vascular growth rates were < 3% of those in control animals receiving either Ringer's solution or bovine trypsin inhibitor (Trasylol). Subconjunctival B16 melanoma implants in mice receiving the inhibitor weight < 2.5% of implants in mice receiving Ringer's solution, Trasylol, or albumin. Histologic study of major organs and standard blood tests revealed no toxic effects in any of the animals. The inhibitor did not retard the growth of either tumor cell type in tissue culture at concentrations as high as 1 mg/ml. These results suggest that the cartilage factor does not interfere with the growth of the tumor cell population directly but that it prevents tumor growth by inhibiting angiogenesis.
在两种不同的动物模型中，通过局部输注部分纯化的软骨提取物，血管生成和肿瘤生长受到抑制。在植入V2癌角膜并接受抑制剂的兔子中，血管生长速率低于接受林格氏液或牛胰蛋白酶抑制剂（抑肽酶）的对照动物的3%。接受抑制剂的小鼠结膜下B16黑色素瘤植入物的重量小于接受林格氏液、抑肽酶或白蛋白的小鼠植入物重量的2.5%。对主要器官的组织学研究和标准血液检测显示，所有动物均未出现毒性作用。在浓度高达1mg/ml时，该抑制剂在组织培养中不会抑制任何一种肿瘤细胞类型的生长。这些结果表明，软骨因子不会直接干扰肿瘤细胞群体的生长，而是通过抑制血管生成来阻止肿瘤生长。
