Kruse V, Lind O
Scand J Clin Lab Invest. 1977 Apr;37(2):149-54. doi: 10.1080/00365517709156070.
A radioimmunoassay for the measurement of total thyroxine in unextracted serum or plasma is described. The assay is performed according to the sequential saturation principle in less than 4 h. The entire assay is carried out at room temperature. An antiserum of high affinity was used as binder, ammonium 8-anilino-1-naphthalene-sulphonate as displacing agent, and activated charcoal as adsorbent in the separation step. The standard curve was linear in the range 0-136 nmol/l serum. The precision in terms of SD was nearly the same through-out this range. Estimates of within-assay SD varied from 1.3 to 4.1 and between-assay SD from 0 to 3.3 nmol/l. The recovery of thyroxine added to normal plasma was 100% and the sensitivity was 6 nmol/l. Mean thyroxine concentration in plasma from young bulls was 92 nmol/l. The assay appears to be very well suited for experimental purposes when high precision is essential.
本文描述了一种用于测定未提取血清或血浆中总甲状腺素的放射免疫分析方法。该分析方法按照顺序饱和原理进行，耗时不到4小时。整个分析过程在室温下进行。在分离步骤中，使用高亲和力的抗血清作为结合剂，8-苯胺基-1-萘磺酸铵作为置换剂，活性炭作为吸附剂。标准曲线在血清浓度0 - 136 nmol/l范围内呈线性。在此范围内，以标准差表示的精密度几乎相同。批内标准差估计值在1.3至4.1之间，批间标准差在0至3.3 nmol/l之间。添加到正常血浆中的甲状腺素回收率为100%，灵敏度为6 nmol/l。年轻公牛血浆中甲状腺素的平均浓度为92 nmol/l。当需要高精度时，该分析方法似乎非常适合实验目的。
