血清3,3',5' -（反式）三碘甲状腺原氨酸的测定及其来源注释

Measurement of serum 3,3',5'-(reverse) T3, with comments on its derivation.

作者信息

Griffiths R S, Black E G, Hoffenberg R

出版信息

Clin Endocrinol (Oxf). 1976 Nov;5(6):679-85. doi: 10.1111/j.1365-2265.1976.tb03871.x.

DOI:10.1111/j.1365-2265.1976.tb03871.x

Abstract

A radioimmunoassay for the measurement of l-3,3',5'-triiodothyronine (reverse TO, rT3) has been developed for use with unextracted serum. The highly specific antiserum showed no cross-reactivity with l-3,3'5-triiodothyronine (T3) or tetradiodothyroacetic acid (T4A) and cross-reaction with L-thyroxine (T4) was low enough to be discounted for routine assay purposes. If a normal amount of rT3 was added to serum T4 cross-reactivity decreased considerably. Serial dilutions of hyperthyroid sera gave dose-response curves which were parallel to the rT3 standard curve. Serum concentrations of rT3 (mean+/- SEM) were 0-68 +/- 0-02 nmo1/1 in sixty-seven normal subjects, 0-19 +/- 0-02 nmo1/1 in twelve hypothyroid patients and 1-18 +/- 0-12 nmo1/1 in seventeen hyperthyroid patients. In sixteen patients with TO-toxicosis rTO was 0-42 +/- 0-04 nmo1/1 and eighteen patients with high circulating TBG had a mean rT3 of 0-54 +/- 0-03 nmo1/1.

摘要

已开发出一种用于测量L-3,3',5'-三碘甲状腺原氨酸（反三碘甲状腺原氨酸，rT3）的放射免疫分析法，可用于未经提取的血清。这种高度特异性的抗血清与L-3,3',5'-三碘甲状腺原氨酸（T3）或四碘甲状腺乙酸（T4A）无交叉反应，与L-甲状腺素（T4）的交叉反应低到在常规检测中可忽略不计。如果向血清中加入正常量的rT3，T4交叉反应会显著降低。甲亢血清的系列稀释给出的剂量反应曲线与rT3标准曲线平行。67名正常受试者的血清rT3浓度（均值±标准误）为0.68±0.02nmol/L，12名甲状腺功能减退患者为0.19±0.02nmol/L，17名甲亢患者为1.18±0.12nmol/L。16名T3中毒患者的rT3为0.42±0.04nmol/L，18名循环中甲状腺素结合球蛋白（TBG）水平高的患者的平均rT3为0.54±0.03nmol/L。