Nye L, Hassan M, Willmott E, Landon J
J Clin Pathol. 1976 May;29(5):452-7. doi: 10.1136/jcp.29.5.452.
A simple radioimmunoassay has been developed for service purposes to determine serum total thyroxine levels. Only three additions are required, of standard or sample, labelled thyroxine and antibody in polyethylene glycol. After 2 hours' incubation at room temperature the antibody-bound and free fractions are separated by centrifugation. Serum total thyroxine levels were measured in 195 euthyroid subjects and it was established that normal values lay within the range 57 to 155 nmol/1. Serial blood samples taken over a 24-hour period, from 11 subjects, indicated that there was no circadian rhythm so that samples for total thyroxine assay can be taken at any time of the day. Similar results were obtained using serum or plasma. Satisfactory results were obtained for three quality control sera when measured by seven different laboratories using this method.
已开发出一种简单的放射免疫测定法用于常规检测血清总甲状腺素水平。只需添加三次，即添加标准品或样品、标记甲状腺素以及聚乙二醇中的抗体。在室温下孵育2小时后，通过离心分离结合抗体的部分和游离部分。对195名甲状腺功能正常的受试者测定了血清总甲状腺素水平，确定正常值范围为57至155纳摩尔/升。从11名受试者身上采集的24小时连续血样表明，不存在昼夜节律，因此总甲状腺素检测的样本可在一天中的任何时间采集。使用血清或血浆获得了相似结果。七个不同实验室使用该方法对三种质量控制血清进行检测时均获得了满意结果。
