Brozman M
Acta Histochem. 1980;67(1):80-5. doi: 10.1016/S0065-1281(80)80057-2.
In histological preparations commonly fixed in formaldehyde and embedded in paraffin which on immunofluorescent examination yielded results negative results or but very poor immunofluorescence, prominently positive results were obtained by chymotrypsin treatment on using the method of fluorescent antibodies. Corresponding results were also obtained in cryostat sections fixed for some min up to several days in formaldehyde so that immunofluorescence was completely negative. The positive immunofluorescent results indicate total or partial restoration of antigenicity of tissue proteins previously altered by formaldehyde.
在通常用甲醛固定并包埋在石蜡中的组织学标本中，经免疫荧光检查结果为阴性或免疫荧光非常差，但采用荧光抗体法经胰凝乳蛋白酶处理后得到了显著的阳性结果。在甲醛中固定几分钟至几天的低温切片中也得到了相应结果，以至于免疫荧光完全为阴性。阳性免疫荧光结果表明先前被甲醛改变的组织蛋白的抗原性得到了全部或部分恢复。
