Brozman M
Acta Histochem. 1978;63(2):251-60. doi: 10.1016/S0065-1281(78)80032-4.
Immunohistochemical studies of paraffin sections of formaldehyde-fixed material and formaldehyde-fixed cryostat sections treated with trypsin or other enzymes were undertaken. Formaldehyde treatment resulted mostly in partial or total loss of antigenicity, as visualized by fluorescent antibody technique in tissue sections. The digestion of sections with trypsin resulted usually in total or partial restitution of antigenicity, previously altered with formaldehyde. This effect is suggested to consist primarily in breaking formaldehyde-induced intermolecular cross-links. Restitution of antigenicity altered by formaldehyde can also be achieved on using pepsin or other proteolytic enzymes, or by some other technique. When the effect of one enzyme proves insufficient, it can readily be complemented by the action of another enzyme applied subsequently. Mild trypsin treatment preserves well the morphological structure of most tissues.
对甲醛固定材料的石蜡切片以及经胰蛋白酶或其他酶处理的甲醛固定低温恒温器切片进行了免疫组织化学研究。甲醛处理大多导致抗原性部分或完全丧失，这在组织切片的荧光抗体技术中可见。用胰蛋白酶消化切片通常会使先前因甲醛而改变的抗原性全部或部分恢复。这种效应主要被认为是打破了甲醛诱导的分子间交联。使用胃蛋白酶或其他蛋白水解酶，或通过其他一些技术，也可以实现被甲醛改变的抗原性的恢复。当一种酶的效果证明不足时，随后应用的另一种酶的作用可以很容易地对其进行补充。温和的胰蛋白酶处理能很好地保留大多数组织的形态结构。
