Brozman M
Cesk Patol. 1979 Nov;15(4):215-20.
Immunohistochemical methods were employed for the investigation of bioptic and necroptic material fixed in formol and embedded in paraffin after it had produced very little or no fluorescence at all in the use of routine immunofluorescence techniques. Partial digestion with pepsin or trypsin or both enzymes in succession mostly facilitated positive immunohistological reaction. This particular procedure gives more scope to the methodological possibilities of histological material examination in that it offers an opportunity for the immunohistological investigation of even old material not specially preserved for immunohistological purposes but routinely fixed in ordinary formol. The simplification adds general availability to the method.
对于使用常规免疫荧光技术时荧光很少或根本没有荧光的活检和坏死材料，采用免疫组织化学方法进行研究。用胃蛋白酶或胰蛋白酶或两种酶相继进行部分消化，大多有助于产生阳性免疫组织学反应。这一特殊程序为组织学材料检查的方法可能性提供了更多空间，因为它为即使是未专门为免疫组织学目的保存、而是常规用普通甲醛固定的陈旧材料进行免疫组织学研究提供了机会。这种简化使该方法更具通用性。
