Iskander A R, Jørgensen R J
Acta Vet Scand. 1980;21(3):330-5. doi: 10.1186/BF03546864.
A morphological description was given of the infective stage of the cattle lungworm Dictyocaulus viviparus. The larvae were stained with iodine and examined under an ordinary microscope after isolation with the bileagar technique used for processing herbage samples. Under these conditions the internal structures appeared of limited value to the identification. The larvae were characterized by their measurements as well as by their shape and profile. A comparison between larvae isolated from the field and larvae harvested from faecal cultures revealed that field larvae were smaller and more uniform in size. Of the reported characteristics the typical profile, the comparatively small size (range 305–400 µm), and the retaining of iodine stain were found most useful in routine recognition of D. viviparus infective larvae in isolates from herbage samples containing free living nematode species as well as infective stages of parasitic species.
对牛肺线虫胎生网尾线虫的感染阶段进行了形态学描述。幼虫用碘染色，并在用用于处理草本植物样本的胆汁琼脂技术分离后，在普通显微镜下进行检查。在这些条件下，内部结构对于鉴定的价值有限。幼虫通过其测量数据以及形状和轮廓来表征。对从野外分离的幼虫和从粪便培养物中收获的幼虫进行比较发现，野外幼虫较小且大小更均匀。在所报道的特征中，典型轮廓、相对较小的尺寸（范围为305 - 400微米）以及碘染色的保留，被发现对于常规识别来自含有自由生活线虫物种以及寄生物种感染阶段的草本植物样本分离物中的胎生网尾线虫感染性幼虫最为有用。
