Lucero M A, Fridman W H, Provost M A, Billardon C, Pouillart P, Dumont J, Falcoff E
Cancer Res. 1981 Jan;41(1):294-9.
Natural killer cell activity, which represents the spontaneous cytotoxicity of lymphocytes toward tumor cells, has been measured in 173 tumor-bearing patients and 25 healthy volunteers; no significant difference was found in mean natural killer cell activity between the two groups. The parameters of interferon-induced activation of natural killer cells were studied in order to provide a suitable test for monitoring the effect of interferon in clinical trials. The three interferons tested (leukocyte, lymphoblastoid, and fibroblast) were equally active in inducing spontaneous cytotoxicity of lymphocytes from all healthy individuals and tumor-bearing patients studied. Incubation for one hr with 100 units of interferon was sufficient to increase spontaneous cytotoxicity activity, the maximum effect being obtained when lymphocytes were incubated with 1000 units of any of the interferons used. This effect was blocked with the appropriate antiinterferon sera. The target cells for interferon seem to be positive Fc gamma receptor lymphocytes.
自然杀伤细胞活性代表淋巴细胞对肿瘤细胞的自发细胞毒性，已在173例肿瘤患者和25名健康志愿者中进行了测量；两组之间的平均自然杀伤细胞活性未发现显著差异。为了提供一种合适的试验来监测干扰素在临床试验中的效果，对干扰素诱导的自然杀伤细胞活化参数进行了研究。所测试的三种干扰素（白细胞干扰素、淋巴母细胞干扰素和成纤维细胞干扰素）在诱导所有研究的健康个体和肿瘤患者的淋巴细胞自发细胞毒性方面具有同等活性。用100单位干扰素孵育1小时足以增加自发细胞毒性活性，当淋巴细胞与所用任何一种干扰素的1000单位孵育时可获得最大效果。这种作用被相应的抗干扰素血清阻断。干扰素的靶细胞似乎是Fcγ受体阳性淋巴细胞。
