Haddow J E, Morin M E, Holman M S, Miller W A
Clin Chem. 1981 Jan;27(1):61-3.
A single-blind study involving amniotic-fluid samples from 214 pregnancies of known outcome confirms that an electrophoretically distinct isoenzyme of acetylcholinesterase is associated with fetal open neural tube defects. Furthermore, only one of 13 amniotic-fluid samples with false-positive results for alpha-fetoprotein showed the characteristic isoenzyme, indicating that qualitative acetylcholinesterase assessment can decrease the proportion of false positives from the alpha-fetoprotein assay. We have also identified this characteristic isoenzyme in amniotic fluids from pregnancies in which other serious fetal defects occurred. A detailed electrophoresis protocol for identifying this characteristic isoenzyme is described.
一项针对214例已知妊娠结局的羊水样本的单盲研究证实，一种电泳性质不同的乙酰胆碱酯酶同工酶与胎儿开放性神经管缺陷有关。此外，在13份甲胎蛋白检测结果为假阳性的羊水样本中，只有一份显示出这种特征性同工酶，这表明乙酰胆碱酯酶的定性评估可以降低甲胎蛋白检测假阳性的比例。我们还在发生其他严重胎儿缺陷的妊娠羊水中鉴定出了这种特征性同工酶。本文描述了一种用于鉴定这种特征性同工酶的详细电泳方法。
