Sandoz P A, Meier E
Stain Technol. 1978 Jul;53(4):195-7. doi: 10.3109/10520297809111465.
The Klüver-Barrera procedure, using luxol fast blue and cresyl violet for a combined nissl and myelin stain, was adapted to unfixed cryostat sections. Neuronal nucleoli appeared as distinct dark blue structures. The color contrast between violet Nissl substance and the nucleoli facilitated their recognition in human and in rat central nervous systems. This modified staining procedure enabled us to combine a counting of nerve cells with a histochemical investigation by applying each technique to a different set of sections cut from the same block of unfixed, frozen brain tissue.
克吕弗-巴雷拉染色法使用鲁戈氏坚牢蓝和甲酚紫进行尼氏和髓鞘联合染色，适用于未固定的低温恒温器切片。神经元核仁呈现为明显的深蓝色结构。紫色的尼氏物质与核仁之间的颜色对比有助于在人类和大鼠中枢神经系统中识别它们。这种改良的染色方法使我们能够通过将每种技术应用于从同一块未固定的冷冻脑组织切下的不同切片组，将神经细胞计数与组织化学研究结合起来。
