Vanroelen C, Vakaet L, Andries L
Anat Embryol (Berl). 1980;160(3):361-7. doi: 10.1007/BF00305114.
The distribution of alcian blue (AB) positivity, and its sensitivity to streptococcal and testicular hyaluronidase, were studied in primitive streak stage chick blastoderms. Accumulation of hyaluronate was observed in deep layer (DL) cells and on laterally migrating middle layer (ML) cells. During the formation of the middle layer, a first stage, namely de-epithelialization of the upper layer cells, is recognized and correlated with the absence of hyaluronate. A second stage, namely migration of the de-epithelialized upper layer cells laterally to the edge of the area pellucida, is correlated with the accumulation of AB-positivity. The AB-staining also demonstrated the accumulation of both sulphated and not-sulphated mucopolysaccharides, where a basal lamina is present.
研究了原条期鸡胚盘中阿尔新蓝（AB）阳性的分布及其对链球菌和睾丸透明质酸酶的敏感性。在深层（DL）细胞和侧向迁移的中层（ML）细胞中观察到透明质酸盐的积累。在中层形成过程中，识别出第一阶段，即上层细胞的去上皮化，这与透明质酸盐的缺失相关。第二阶段，即去上皮化的上层细胞侧向迁移到明区边缘，与AB阳性的积累相关。AB染色还显示在存在基膜的地方，硫酸化和非硫酸化粘多糖都有积累。
