Vanroelen C, Vakaet L, Andries L
J Embryol Exp Morphol. 1980 Apr;56:169-78.
Acid mucopolysaccharides in the extracellular compartment of early chick blastoderms (16 h of incubation) were labelled with tritiated glucosamine and/or [35S]sulphate. The incorporation pattern was studied autoradiographically. Treatment with testicular hyaluronidase revealed a testicular hyaluronidase-sensitive fraction, mainly at the periphery of Middle Layer and Deep Layer cells, and a testicular hyaluronidase-resistant fraction, mainly at the ventral side of the Upper Layer. A biochemical analysis, utilizing chondroitinase ABC and nitrous acid, followed by cellulose acetate electrophoresis, demonstrated the synthesis of a non-sulphated fraction, i.e. hyaluronic acid and/or chondroitin, and a sulphated fraction, comprising two undersulphated components, i.e. chondroitin sulphate, and heparan sulphate or heparin. The appearance of different AMPS in specific areas of the early chick blastoderm is regarded as an early specialization of the extracellular compartment.
用氚标记的葡糖胺和/或[35S]硫酸盐对早期鸡胚盘（孵化16小时）细胞外区室中的酸性粘多糖进行标记。通过放射自显影研究掺入模式。用睾丸透明质酸酶处理后发现，存在一个对睾丸透明质酸酶敏感的部分，主要位于中层和深层细胞的周边，以及一个对睾丸透明质酸酶抗性的部分，主要位于上层的腹侧。利用软骨素酶ABC和亚硝酸进行生化分析，随后进行醋酸纤维素电泳，结果表明合成了一种非硫酸化部分，即透明质酸和/或软骨素，以及一种硫酸化部分，该部分包含两种硫酸化不足的成分，即硫酸软骨素和硫酸乙酰肝素或肝素。早期鸡胚盘特定区域中不同酸性粘多糖的出现被视为细胞外区室的早期特化。
