Cassiman J J, van Leuven F, van der Schueren B, van den Berghe H
Cell Tissue Res. 1980;213(2):301-10. doi: 10.1007/BF00234789.
The localization of alpha 2macroglobulin (alpha 2M) has been examined by an indirect immunofluorescent technique in frozen sections of various human tissues. The results indicate that alpha 2M is present only in connective tissues and blood. The outer medulla of the kidney and the submucosa of the gut showed the strongest reaction. Epithelia or endothelial cells were unreactive. In liver, only the Kupffer cells were stained. These results were confirmed withe the immunoperoxidase technique and by the study of tissue extracts in crossed immunoelectrophoresis (CIE). As a positive control a polyspecific antiserum prepared against whole human fibroblasts as well as anti-albumin were used. Our findings are interpreted in the light of the observations that alpha 2M is synthesized and selectively ingested by fibroblasts.
已通过间接免疫荧光技术在各种人体组织的冰冻切片中检测了α2巨球蛋白（α2M）的定位。结果表明，α2M仅存在于结缔组织和血液中。肾外髓质和肠道黏膜下层反应最强。上皮细胞或内皮细胞无反应。在肝脏中，仅库普弗细胞被染色。这些结果通过免疫过氧化物酶技术以及交叉免疫电泳（CIE）中的组织提取物研究得到证实。作为阳性对照，使用了针对全人成纤维细胞制备的多特异性抗血清以及抗白蛋白。根据α2M由成纤维细胞合成并被其选择性摄取的观察结果来解释我们的发现。
