Saito K, Natsuno T, Mitsuhashi S
Immunology. 1980 Dec;41(4):937-45.
T- and B-cell participation in the immune response induced by immune ribonucleic acid (iRNA) preparations against T-dependent antigens was studied using athymic nude, neonatally thymectomized (NT) and cyclophosphamide-treated (CY) mice. The iRNA(T + B) preparations were made from the spleen of BALB/c mice immunized with these antigens. Injection of the iRNA into nude or NT mice caused an increase in the number of specific rosette-forming cells (RFC) and of memory cells capable of responding to secondary stimulus with a small dose of the corresponding antigen. Injection with T-dependent antigens or with iRNA(T + B) did not cause any immune response in CY mice, suggesting depletion of the B-cell function. The iRNA(T) and iRNA(B) were prepared, respectively, from the thymuses of BALB/c mice and from the spleens of nude mice which had been immunized with T-dependent antigens. Injection of nude mice with both iRNA(T) and iRNA(B) caused an increase in the number of specific RFC and the secondary antibody formation response after boosting with a small dose of the corresponding antigen. Injection of iRNA(T) preparation into nude mice could induce the anamnestic response after boosting. However, neither of the iRNA(T) or iRNA(B) preparation could induce in nude mice the proliferation of the number of specific RFC. These results indicate the presence of at least two kinds of iRNA preparations against T-dependent antigens and that the cooperation of iRNA(T) and iRNA(B) was required for the induction of immune response against T-dependent antigens.
利用无胸腺裸鼠、新生期胸腺切除（NT）小鼠和环磷酰胺处理（CY）小鼠，研究了T细胞和B细胞在免疫核糖核酸（iRNA）制剂诱导的针对T细胞依赖性抗原的免疫反应中的参与情况。iRNA（T + B）制剂由用这些抗原免疫的BALB/c小鼠的脾脏制备。将iRNA注射到裸鼠或NT小鼠中会导致特异性玫瑰花结形成细胞（RFC）数量以及能够对小剂量相应抗原的二次刺激作出反应的记忆细胞数量增加。注射T细胞依赖性抗原或iRNA（T + B）在CY小鼠中未引起任何免疫反应，提示B细胞功能耗竭。iRNA（T）和iRNA（B）分别由用T细胞依赖性抗原免疫的BALB/c小鼠的胸腺和裸鼠的脾脏制备。给裸鼠同时注射iRNA（T）和iRNA（B）会导致特异性RFC数量增加以及在用小剂量相应抗原加强免疫后产生二次抗体形成反应。将iRNA（T）制剂注射到裸鼠中在加强免疫后可诱导回忆反应。然而，iRNA（T）或iRNA（B）制剂均不能在裸鼠中诱导特异性RFC数量的增殖。这些结果表明存在至少两种针对T细胞依赖性抗原的iRNA制剂，并且诱导针对T细胞依赖性抗原的免疫反应需要iRNA（T）和iRNA（B）的协同作用。
