Maehara N, Komatsu H, Shimoda K, Makino S, Nagano Y, Matumoto M
Microbiol Immunol. 1980;24(10):907-14. doi: 10.1111/j.1348-0421.1980.tb02896.x.
The production of the virus-inhibiting factor or interferon (IF) was highest in cells incubated at 37 C after inoculation with Newcastle disease (ND) virus and decreased as the incubation temperature was lowered. Shift-down of incubation temperature to 32 C or 34 C after incubation at 37 C for 4--7 hr enhanced IF production in cell cultures stimulated with ND virus, as compared with cultures incubated continuously at 37 C. Shift-down to 32 C after incubation at 37 C for 6 hr was optimal for this enhancement of IF yield. Enhanced IF production was also observed in cell cultures irradiated by ultraviolet light 4--7 hr after stimulation with ND virus.
接种新城疫（ND）病毒后，在37℃培养的细胞中，病毒抑制因子即干扰素（IF）的产量最高，且随着培养温度降低而减少。在37℃培养4 - 7小时后，将培养温度下调至32℃或34℃，与在37℃持续培养的细胞培养物相比，可增强经ND病毒刺激的细胞培养物中IF的产生。在37℃培养6小时后下调至32℃对这种IF产量的提高最为有利。在用ND病毒刺激4 - 7小时后经紫外线照射的细胞培养物中也观察到IF产量增加。
