Glass J, Wertz G W
Nucleic Acids Res. 1980 Dec 11;8(23):5739-51. doi: 10.1093/nar/8.23.5739.
A significant difference was found to exist in the number of bases per unit length of single-stranded RNA as compared to single-stranded DNA when single-stranded RNA or DNA molecules of known nucleotide sequence were measured by electron microscopy using a cytochrome spreading technique. Using this technique, single-stranded RNA was found to have 17.5% more bases per unit of length than single-stranded DNA. These ratios were verified using four different denaturing conditions for the RNA: 80% formamide, 80% formamide plus glyoxal, 80% formamide/4M urea and 80% formamide/4M urea plus glyoxal. Molecules ranging in size from 1541 to 5386 nucleotides were examined and the number of bases per unit length was found to vary inversely with micrometer was consistent when RNA and DNA molecules of the same length were compared.
当使用细胞色素铺展技术通过电子显微镜测量已知核苷酸序列的单链RNA或DNA分子时，发现单链RNA每单位长度的碱基数与单链DNA相比存在显著差异。使用该技术发现，单链RNA每单位长度的碱基比单链DNA多17.5%。这些比率在RNA的四种不同变性条件下得到验证：80%甲酰胺、80%甲酰胺加乙二醛、80%甲酰胺/4M尿素和80%甲酰胺/4M尿素加乙二醛。对大小从1541到5386个核苷酸的分子进行了检查，发现每单位长度的碱基数与微米成反比，当比较相同长度的RNA和DNA分子时这是一致的。
