Crampton J, Humphries S, Woods D, Williamson R
Nucleic Acids Res. 1980 Dec 20;8(24):6007-17. doi: 10.1093/nar/8.24.6007.
Poly(A)+ RNA populations derived from normal lymphocytes and fibroblasts have been compared by hybridising each RNA to cDNA derived from the other RNA population. This indicated that approximately 75% of the sequences were common to both, and that these were present at different concentrations in the two cell types. The two RNA populations were further compared by hybridising them to a cDNA recombinant library derived from lymphocyte poly(A)+ RNA. This allowed the identification of clones containing sequences which are abundant in lymphocyte poly(A)+ RNA but absent or rare in fibroblast poly(A)+ RNA. A direct estimation of the abundance of five of these sequences in lymphocyte cDNA demonstrated that clones can be detected by such a procedure if they represent 0.2% or greater of the original cDNA population.
通过将来自正常淋巴细胞和成纤维细胞的聚腺苷酸加尾（Poly(A)+）RNA群体分别与源自另一种RNA群体的互补DNA（cDNA）杂交，对它们进行了比较。这表明大约75%的序列在两者中是共有的，并且这些序列在两种细胞类型中的浓度不同。通过将这两个RNA群体与源自淋巴细胞聚腺苷酸加尾RNA的cDNA重组文库杂交，进一步对它们进行了比较。这使得能够鉴定出包含在淋巴细胞聚腺苷酸加尾RNA中丰富但在成纤维细胞聚腺苷酸加尾RNA中不存在或稀少的序列的克隆。对淋巴细胞cDNA中其中五个序列的丰度进行直接估计表明，如果这些克隆代表原始cDNA群体的0.2%或更多，则可以通过这种方法检测到它们。
Zotero 插件
