脑微管蛋白和肌动蛋白cDNA序列：重组质粒的分离
Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.
作者信息
Ginzburg I, de Baetselier A, Walker M D, Behar L, Lehrach H, Frischauf A M, Littauer U Z
出版信息
Nucleic Acids Res. 1980 Aug 25;8(16):3553-64. doi: 10.1093/nar/8.16.3553.
Abstract
Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322 plasmid and transformation of E. coli chi 1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.
摘要
富含微管蛋白和肌动蛋白序列的大鼠脑信使核糖核酸用于制备双链互补脱氧核糖核酸。通过将双链脱氧核糖核酸插入pBR322质粒的Pst1位点并转化大肠杆菌chi 1776宿主，构建了重组克隆文库。鉴定并表征了携带编码微管蛋白和肌动蛋白序列的克隆。
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