Kronenberg M, Kraig E, Siu G, Kapp J A, Kappler J, Marrack P, Pierce C W, Hood L
J Exp Med. 1983 Jul 1;158(1):210-27. doi: 10.1084/jem.158.1.210.
We attempted to determine whether T cells express any VH gene segments. cDNA libraries were constructed from one suppressor and two helper T cell hybridomas. Both the library construction and screening were designed to maximize detection of a wide range of VH gene segments. One screening method should detect about half of the sequenced VH genes, while the second should detect most of these genes. The probability of detecting a VH gene homologous to the probes and present at 10 copies per cell was 77% for one helper cell cDNA library, 88% for the second helper cell library, and greater than 99% for the suppressor cell library. No cDNA clones with VH gene segments were detected. From this result, we conclude that VH gene segments are not likely to encode the antigen-specific receptor in the cells we tested.
我们试图确定T细胞是否表达任何重链可变区（VH）基因片段。从一个抑制性T细胞杂交瘤和两个辅助性T细胞杂交瘤构建了互补DNA（cDNA）文库。文库构建和筛选的设计目的都是为了最大程度地检测广泛的VH基因片段。一种筛选方法应能检测出约一半已测序的VH基因，而另一种方法应能检测出这些基因中的大多数。对于一个辅助性T细胞cDNA文库，检测到与探针同源且每个细胞中有10个拷贝的VH基因的概率为77%，第二个辅助性T细胞文库为88%，抑制性T细胞文库则大于99%。未检测到带有VH基因片段的cDNA克隆。根据这一结果，我们得出结论，在所测试的细胞中，VH基因片段不太可能编码抗原特异性受体。
