Hyltoft Petersen P, Felding P, Hørder M, Tryding N
Scand J Clin Lab Invest. 1980;40(7):623-8. doi: 10.3109/00365518009091973.
The effect of posture on changes in protein composition of serum and its dependence on molecular size has been investigated in forty individuals classified by age and sex. Blood specimens were drawn (A) at 0700 hours with persons still resting in bed after an overnight sleep; (B) at 0815 hours, in sitting position after 1 h of moderate exercise and again (C) at 0915 hours, lying in bed after 1 h of recumbency. Concentrations of alpha 1-antitrypsin, albumin and alpha 2-macroglobulin in serum were determined with high analytical precision. The mean fractional increases of concentrations from lying (A) to sitting (B) were 0.069 for alpha 1-antitrypsin and 0.063 for albumin, but lower, 0.040, for alpha 2-macroglobulin. Thus these increases, being related to molecular size, were not simply caused by posture induced changes of plasma volume. Considering the results of each individual we found a constant difference between the increase of alpha 1-antitrypsin and alpha 2-macroglobulin of about 0.030 for the whole range of increases (i.e. for alpha 1-antitrypsin 0-0.15). In contrast, the effects of recumbency were independent of molecular size. The mean, fractional decreases in concentration induced by changing of position from sitting (B) to lying (C) were approximately 0.065 for all three proteins.
研究了姿势对血清蛋白质组成变化的影响及其与分子大小的关系，研究对象为40名按年龄和性别分类的个体。采集血样：(A) 在早上7点，受试者经过一夜睡眠后仍卧床休息时；(B) 在早上8点15分，进行1小时适度运动后坐着时；以及(C) 在早上9点15分，平躺1小时后卧床时。以高分析精度测定血清中α1-抗胰蛋白酶、白蛋白和α2-巨球蛋白的浓度。从平躺状态(A)到坐姿状态(B)，α1-抗胰蛋白酶浓度的平均分数增加为0.069，白蛋白为0.063，但α2-巨球蛋白较低，为0.040。因此，这些增加与分子大小有关，并非仅仅由姿势引起的血浆容量变化所致。考虑每个个体的结果，我们发现在整个增加范围内（即α1-抗胰蛋白酶为0 - 0.15），α1-抗胰蛋白酶和α2-巨球蛋白增加量之间的恒定差异约为0.030。相比之下，平躺的影响与分子大小无关。从坐姿状态(B)变为平躺状态(C)所引起的所有三种蛋白质浓度的平均分数下降约为0.065。
