Morimoto C, Abe T, Toguchi T, Homma M
Tohoku J Exp Med. 1980 Apr;130(4):321-34. doi: 10.1620/tjem.130.321.
A rabbit antiserum to human fetal brain after multiple absorption reacted with 100% of thymocytes, 55% of peripheral blood lymphocytes and 90% of enriched T lymphocytes, but not significantly with B lymphocytes. Spontaneous SRBC rosette formation was inhibited by anti-BAT pretreatment, but EAC-rosette formation remained unaffected. The antiserum was itself highly stimulatory. However, cells treated with the antiserum and complement exhibited marked inhibition of responsiveness to Con A, little effect with PHA and no alteration with PWM. The MLC reaction was inhibited only when the responder cells were treated with the antiserum and complement. Treatment of sensitized lymphocytes with the antiserum and complement caused a dose-dependent suppression of blastogenic response to both PPD and n-DNA. No effect, however, was noted in MIF producing cells. Con A induced suppressor function of lymphocytes was abolished by treatment with the antiserum and complement. These results indicate that the anti-BAT serum obtained by us can be utilized for the isolation of T lymphocyte subsets.
经过多次吸收的抗人胎脑兔抗血清与100%的胸腺细胞、55%的外周血淋巴细胞和90%的富集T淋巴细胞发生反应，但与B淋巴细胞的反应不明显。抗BAT预处理可抑制自发绵羊红细胞玫瑰花结形成，但EAC玫瑰花结形成不受影响。该抗血清本身具有高度刺激性。然而，用抗血清和补体处理的细胞对刀豆球蛋白A的反应性受到明显抑制，对植物血凝素影响不大，对美洲商陆丝裂原无改变。只有当反应细胞用抗血清和补体处理时，混合淋巴细胞反应才受到抑制。用抗血清和补体处理致敏淋巴细胞会导致对结核菌素纯蛋白衍生物和天然DNA的增殖反应呈剂量依赖性抑制。然而，在产生巨噬细胞移动抑制因子的细胞中未观察到影响。用抗血清和补体处理可消除刀豆球蛋白A诱导的淋巴细胞抑制功能。这些结果表明，我们获得的抗BAT血清可用于分离T淋巴细胞亚群。
