Wicher V, Amesty C
Immunol Commun. 1982;11(4):303-14. doi: 10.3109/08820138209050730.
Antisera to human brain (AHBS) and human thymocytes (AHTS) were produced in rabbits and selectively absorbed to render them specific for T cells. After absorption AHBS, but not AHTS, lost most of its cytotoxic activity against T cells. Absorbed AHBS bound up to 95% of peripheral blood T lymphocytes as detected by indirect immunofluorescence and inhibited up to 46% of the lytic activity of AHTS; however, it was incapable of inhibiting the E-rosette formation of T lymphocytes. All 10 samples of human peripheral blood lymphocytes, pretreated with AHBS, were significantly suppressed in their response to antigens, but fewer samples were affected in their response to mitogens and to allogeneic stimulation, indicating diversity in the nature of the receptors involved in the cellular responses.
用人脑（AHBS）和人胸腺细胞（AHTS）在兔体内制备抗血清，并进行选择性吸收使其对T细胞具有特异性。吸收后，AHBS而非AHTS失去了对T细胞的大部分细胞毒性活性。通过间接免疫荧光检测，吸收后的AHBS可结合高达95%的外周血T淋巴细胞，并抑制高达46%的AHTS的裂解活性；然而，它无法抑制T淋巴细胞的E花环形成。用AHBS预处理的所有10份人外周血淋巴细胞样本对抗抗原的反应均受到显著抑制，但对抗有丝分裂原和同种异体刺激的反应受影响的样本较少，这表明细胞反应中涉及的受体性质存在差异。
