• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

免疫吸附剂在研究抗体与抹香鲸肌红蛋白及其合成抗原位点结合中的应用。

Use of immunoadsorbents for the study of antibody binding to sperm whale myoglobin and its synthetic antigenic sites.

作者信息

Twining S S, Atassi M Z

出版信息

J Immunol Methods. 1979;30(2):139-51. doi: 10.1016/0022-1759(79)90088-7.

DOI:10.1016/0022-1759(79)90088-7
PMID:91647
Abstract

Conditions for preparing immunoadsorbents of sperm-whale myoglobin and its five synthetic antigenic sites and for desorption of radiolabeled antibodies from the immunoadsorbents were studied. In immunoadsorbent titration studies, the sum of the amounts of antibodies bound in the plateau (maximum binding) by the adsorbents of the five sites accounted quantitatively for the entire (100%) antibody response to sperm-whale myoglobin.

摘要

研究了制备抹香鲸肌红蛋白及其五个合成抗原位点免疫吸附剂的条件，以及从免疫吸附剂上解吸附放射性标记抗体的条件。在免疫吸附剂滴定研究中，五个位点的吸附剂在平台期（最大结合量）结合的抗体量总和在数量上占对抹香鲸肌红蛋白的全部（100%）抗体反应。

相似文献

1
Use of immunoadsorbents for the study of antibody binding to sperm whale myoglobin and its synthetic antigenic sites.免疫吸附剂在研究抗体与抹香鲸肌红蛋白及其合成抗原位点结合中的应用。
J Immunol Methods. 1979;30(2):139-51. doi: 10.1016/0022-1759(79)90088-7.
2
The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species.对肌红蛋白的抗体反应与免疫物种无关。分析了在不同物种中产生的十五种肌红蛋白与抹香鲸肌红蛋白抗血清交叉反应的抗原位点和环境残基中的替换情况。
Biochem J. 1980 Dec 1;191(3):681-97. doi: 10.1042/bj1910681.
3
Antibodies against protein antigenic sites that are identical in the homologous protein of the immunized animal. Autoreactivity in rabbits of antibodies to sperm-whale myoglobin.针对免疫动物同源蛋白中相同蛋白质抗原位点的抗体。兔体内抗抹香鲸肌红蛋白抗体的自身反应性。
Biochim Biophys Acta. 1977 Sep 27;494(1):277-82. doi: 10.1016/0005-2795(77)90156-8.
4
Antigenic structure of sperm whale myoglobin. I. Partition of specificities between antibodies reactive with peptides and native protein.抹香鲸肌红蛋白的抗原结构。I. 与肽和天然蛋白反应的抗体之间特异性的分配
J Immunol. 1982 Jul;129(1):206-11.
5
Genetic control of the immune response to myoglobin. V. Analysis of the cross-reactivity of 12 myoglobins with sperm-whale myoglobin antisera of inbred mouse strains in terms of substitutions in the antigenic sites and in the environmental residues of the sites.对肌红蛋白免疫反应的遗传控制。V. 根据抗原位点及其周围残基的取代情况分析12种肌红蛋白与近交系小鼠品系的抹香鲸肌红蛋白抗血清的交叉反应性。
Immunol Commun. 1981;10(4-5):359-65. doi: 10.3109/08820138109050701.
6
Antibodies to sperm-whale myoglobin evoked by free synthetic peptides of an antigenic site.由抗原位点的游离合成肽引发的针对抹香鲸肌红蛋白的抗体。
Immunol Commun. 1982;11(1):9-16. doi: 10.3109/08820138209050719.
7
Production of monoclonal antibodies with preselected submolecular binding specificities to protein antigenic sites: antibodies to sperm whale myoglobin sites.具有对蛋白质抗原位点预先选定的亚分子结合特异性的单克隆抗体的制备：针对抹香鲸肌红蛋白位点的抗体
Mol Immunol. 1983 Jul;20(7):719-26. doi: 10.1016/0161-5890(83)90049-4.
8
Topographic antigenic determinants recognized by monoclonal antibodies to sperm whale myoglobin.抹香鲸肌红蛋白单克隆抗体识别的拓扑抗原决定簇。
J Biol Chem. 1982 Mar 25;257(6):3189-98.
9
Genetic control of the immune response to myoglobin. IV. Mouse antibodies in outbred and congenic strains against sperm-whale myoglobin recognize the same antigenic sites that are recognized by antibodies raised in other species.对肌红蛋白免疫反应的遗传控制。IV. 远交系和近交系小鼠针对抹香鲸肌红蛋白的抗体识别的抗原位点与其他物种产生的抗体所识别的相同。
Mol Immunol. 1981 May;18(5):447-50. doi: 10.1016/0161-5890(81)90107-3.
10
Production of monoclonal antibodies with pre-selected submolecular binding specificities to protein determinants: demonstration with sperm whale myoglobin.具有对蛋白质决定簇预先选择的亚分子结合特异性的单克隆抗体的制备：以抹香鲸肌红蛋白为例进行说明
Mol Immunol. 1982 Dec;19(12):1699-1702. doi: 10.1016/0161-5890(82)90282-6.

引用本文的文献

1
Subunit interacting surfaces of human hemoglobin in solution: localization of the alpha-beta subunit interacting surfaces on the alpha-chain by a comprehensive synthetic strategy.溶液中人类血红蛋白的亚基相互作用表面：通过综合合成策略确定α-β亚基相互作用表面在α链上的位置。
J Protein Chem. 1999 Feb;18(2):179-85. doi: 10.1023/a:1020623905544.
2
Haemoglobin binding with haptoglobin. Localization of the haptoglobin-binding site on the alpha-chain of human haemoglobin.血红蛋白与触珠蛋白的结合。触珠蛋白结合位点在人血红蛋白α链上的定位。
Biochem J. 1981 Aug 1;197(2):507-10. doi: 10.1042/bj1970507.
3
Binding with lysozyme of antibodies against surface-simulation peptides representing the lysozyme antigenic sites.
针对代表溶菌酶抗原位点的表面模拟肽的抗体与溶菌酶的结合。
Biochem J. 1982 Mar 1;201(3):669-72. doi: 10.1042/bj2010669.
4
Localization and synthesis of the acetylcholine-binding site in the alpha-chain of the Torpedo californica acetylcholine receptor.加州电鳐乙酰胆碱受体α链中乙酰胆碱结合位点的定位与合成
Biochem J. 1984 Dec 15;224(3):995-1000. doi: 10.1042/bj2240995.
5
Localization, synthesis, and activity of an antigenic site on influenza virus hemagglutinin.流感病毒血凝素抗原位点的定位、合成与活性
Proc Natl Acad Sci U S A. 1983 Feb;80(3):840-4. doi: 10.1073/pnas.80.3.840.
6
Structurally inherent antigenic sites. Localization of the antigenic sites of the alpha-chain of human haemoglobin in three host species by a comprehensive synthetic approach.结构上固有的抗原位点。通过综合合成方法在三种宿主物种中定位人血红蛋白α链的抗原位点。
Biochem J. 1982 Apr 1;203(1):201-8. doi: 10.1042/bj2030201.
7
The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species.对肌红蛋白的抗体反应与免疫物种无关。分析了在不同物种中产生的十五种肌红蛋白与抹香鲸肌红蛋白抗血清交叉反应的抗原位点和环境残基中的替换情况。
Biochem J. 1980 Dec 1;191(3):681-97. doi: 10.1042/bj1910681.
8
A novel and comprehensive synthetic approach for the elucidation of protein antigenic structures. Determination of the full antigenic profile of the alpha-chain of human haemoglobin.一种用于阐明蛋白质抗原结构的新颖且全面的合成方法。人血红蛋白α链完整抗原谱的测定。
Biochem J. 1980 Oct 1;191(1):261-4. doi: 10.1042/bj1910261.
9
Precise determination of protein antigenic structures has unravelled the molecular immune recognition of proteins and provided a prototype for synthetic mimicking of other protein binding sites.蛋白质抗原结构的精确测定揭示了蛋白质的分子免疫识别，并为其他蛋白质结合位点的合成模拟提供了原型。
Mol Cell Biochem. 1980 Aug 29;32(1):21-43. doi: 10.1007/BF00421293.
10
Distance calculation of residues neighbouring to lysozyme antigenic sites. Site-neighbouring residues whose evolutionary substitution can modify the characteristics and binding energy of the sites.溶菌酶抗原位点邻近残基的距离计算。其进化替代可改变抗原位点特征和结合能的位点邻近残基。
Biochem J. 1980 Apr 1;187(1):163-72. doi: 10.1042/bj1870163.