Hervonen A, Pickel V M, Joh T H, Reis D J, Linnoila I, Miller R J
Cell Tissue Res. 1981;214(1):33-42. doi: 10.1007/BF00235142.
The human fetal sympathetic ganglia were studied using the indirect peroxidase-antiperoxidase PAP method for immunocytochemical demonstration of three catecholamine-synthesizing enzymes, tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine-N-methyltransferase (PNMT) as well as the neuropeptides leucine (Leu5)-enkephalin and substance P. The neuroblasts of the ganglia showed intense peroxidase immunoreactivity for TH, moderate reaction to DBH, and no reaction to PNMT. The small intensely fluorescent (SIF) cells situated along the blood vessels also showed positive labelling for only two enzymes, TH and BDH. The immunocytochemical localization of these enzymes suggests that both neuroblasts and SIF cells synthesize noradrenalin. Neither the neuroblasts nor SIF cells showed a reaction to substance P, and only the SIF cells contained enkephalin-like immunoreactivity. The role of enkephalin in the noradrenalin-containing SIF cells is unknown, but may be related to neuromodulation of ganglionic transmission.
利用间接过氧化物酶 - 抗过氧化物酶（PAP）法对人胎儿交感神经节进行研究，以免疫细胞化学方法显示三种儿茶酚胺合成酶，即酪氨酸羟化酶（TH）、多巴胺 -β-羟化酶（DBH）和苯乙醇胺 -N-甲基转移酶（PNMT），以及神经肽亮氨酸（Leu5）-脑啡肽和P物质。神经节的神经母细胞对TH呈现强烈的过氧化物酶免疫反应性，对DBH反应中等，对PNMT无反应。沿血管分布的小而强荧光（SIF）细胞也仅对两种酶，即TH和BDH呈阳性标记。这些酶的免疫细胞化学定位表明，神经母细胞和SIF细胞都合成去甲肾上腺素。神经母细胞和SIF细胞对P物质均无反应，只有SIF细胞含有脑啡肽样免疫反应性。脑啡肽在含去甲肾上腺素的SIF细胞中的作用尚不清楚，但可能与神经节传递的神经调节有关。
