Partanen M, Rapoport S I, Reis D J, Joh T H, Stolk J M, Linnoila I, Teitelman G, Hervonen A
Cell Tissue Res. 1984;238(2):217-20. doi: 10.1007/BF00217291.
The catecholamine-synthesizing enzymes, tyrosine hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine-N-methyltransferase were examined by immunohistochemistry in hypertrophied paraganglia of aged male Fischer-344 rats. All paraganglionic cells reacted with antibodies against tyrosine hydroxylase. Dopamine beta-hydroxylase was identified in most paraganglionic cells, indicating that they synthesized norepinephrine. A variable number of paraganglia were positive for phenylethanolamine-N-methyltransferase, which suggested that they synthesized epinephrine. The formaldehyde-induced fluorescence method demonstrated greenish-yellow fluorescence or yellowish-brown fluorescence. The intensity of the fluorescence was in the same range as in adrenal medullary cells. The observations indicate that paraganglia are capable of synthesizing epinephrine.
采用免疫组织化学方法，对老年雄性Fischer-344大鼠增生的副神经节中的儿茶酚胺合成酶、酪氨酸羟化酶、多巴胺β-羟化酶和苯乙醇胺-N-甲基转移酶进行了检测。所有副神经节细胞均与抗酪氨酸羟化酶抗体发生反应。在大多数副神经节细胞中鉴定出多巴胺β-羟化酶，表明它们合成去甲肾上腺素。可变数量的副神经节对苯乙醇胺-N-甲基转移酶呈阳性，这表明它们合成肾上腺素。甲醛诱导荧光法显示出绿黄色荧光或黄棕色荧光。荧光强度与肾上腺髓质细胞中的荧光强度在同一范围内。这些观察结果表明，副神经节能够合成肾上腺素。
