Higgins P J, Borenfreund E
Exp Cell Biol. 1981;49(2):68-77. doi: 10.1159/000163789.
Epithelial cells, isolated from the hepatic tissue of rats bearing carcinogen-induced neoplastic liver nodules, were analyzed by immunological methods for the production of specific liver proteins during alternate in vitro-in vivo passage. Heterotransplantation of several long-term rat liver cell lines into nude mice and subsequent re-establishment in vitro resulted in the derivation of hepatocyte cultures expressing both alpha-fetoprotein and albumin. Prior to passage in vivo these cell lines synthesized by one or neither of these proteins in vitro. Alternate in vitro-in vivo passage thus appears to potentiate tissue-specific protein expression by established liver cell lines.
从携带致癌物诱导的肿瘤性肝结节的大鼠肝脏组织中分离出上皮细胞，通过免疫方法分析其在体外 - 体内交替传代过程中特异性肝脏蛋白的产生。将几种长期大鼠肝细胞系异种移植到裸鼠体内，随后在体外重新建立细胞系，结果得到了同时表达甲胎蛋白和白蛋白的肝细胞培养物。在体内传代之前，这些细胞系在体外仅合成其中一种蛋白或两种蛋白都不合成。因此，体外 - 体内交替传代似乎能增强已建立的肝细胞系的组织特异性蛋白表达。
