Schut H A, Hughes E H, Thorgeirsson S S
In Vitro. 1981 Apr;17(4):275-83. doi: 10.1007/BF02618138.
Radioimmunoassay was used to determine alpha-fetoprotein (AFP), albumin, and transferrin production (ng/10(5) cells/24 h) by two cell lines (7777 and 8994) derived from chemically induced rat hepatomas. alpha-Fetoprotein production was high (2000 to 4400) in 7777, but was very low (0.2 to 0.4) in 8994. Albumin production varied from 0.4-0.8 (7777) to 14-26 (8994). Both lines produced substantial amounts of transferrin (180 to 240 by 7777 and 29 to 42 by 8994). Addition of dimethyl sulfoxide (DMSO, 1 to 4%) or sodium butyrate (BA, 0.5 to 2.0 mM) to the medium inhibited growth in both lines, but 8994 was more sensitive to these agents than 7777. Dimethyl sulfoxide treatment (2 to 4%) resulted in a dose-related decrease (less than 10% of control at 4% DMSO) in AFP, albumin, and transferrin production by 7777, but in 8994, DMSO (1 to 2%) resulted in an increase (up to sixfold) in albumin and transferrin production, without affecting AFP production. By contrast, BA (2 to 4 mM) stimulated the production of all three proteins in both lines, most notably that of albumin (up to sixfold) by 7777 and that of AFP (up to 20-fold) by 8994. It is concluded that both DMSO and BA can enhance the expression of differentiated functions of the hepatoma cell, and that DMSO at the same time can suppress the expression of an oncofetal function. However, neither DMSO nor BA is selective in its effects on specific genes (i.e., normal, adult vs. oncofetal genes), and it appears that their effects may be the result of a more general phenomenon, the expression of which may be related to the stage of differentiation of the cell.
采用放射免疫分析法测定两种源自化学诱导大鼠肝癌的细胞系（7777和8994）的甲胎蛋白（AFP）、白蛋白和转铁蛋白的产量（纳克/10⁵个细胞/24小时）。7777细胞系中甲胎蛋白产量较高（2000至4400），而8994细胞系中产量极低（0.2至0.4）。白蛋白产量在7777细胞系中为0.4 - 0.8，在8994细胞系中为14 - 26。两个细胞系均产生大量转铁蛋白（7777细胞系为180至240，8994细胞系为29至42）。向培养基中添加二甲基亚砜（DMSO，1%至4%）或丁酸钠（BA，0.5至2.0毫摩尔）会抑制两个细胞系的生长，但8994细胞系对这些试剂比7777细胞系更敏感。二甲基亚砜处理（2%至4%）导致7777细胞系的AFP、白蛋白和转铁蛋白产量呈剂量相关下降（4% DMSO时低于对照的10%），但在8994细胞系中，1%至2%的DMSO导致白蛋白和转铁蛋白产量增加（高达六倍），而不影响AFP产量。相比之下，2至4毫摩尔的BA刺激两个细胞系中所有三种蛋白质的产生，最显著的是7777细胞系中白蛋白产量（高达六倍）和8994细胞系中AFP产量（高达20倍）。得出的结论是，DMSO和BA均可增强肝癌细胞分化功能的表达，并且DMSO同时可抑制癌胚功能的表达。然而，DMSO和BA对特定基因（即正常、成人基因与癌胚基因）的作用均无选择性，并且它们的作用似乎可能是一种更普遍现象的结果，这种现象的表达可能与细胞的分化阶段有关。
