Neumann C, Macher E, Sorg C
Immunobiology. 1980 Apr;157(1):12-23. doi: 10.1016/S0171-2985(80)80057-X.
Macrophages were identified to be a major source of interferon produced in murine spleen cell cultures after intravenous injection of Corynebacterium parvum (C. parvum), strain CN 6134 or Bacille Calmette Guérin (BCG). Another strain of C. parvum, CN 5888, which lacks RES stimulating activity and adjuvant activity in vivo, was not effective when injected intravenously. Protein synthesis was required for interferon activity to be produced and protein synthesis was also required for the antiviral state to be expressed. The antiviral activity was relatively stable to pH 2 and neutralized by an antiserum against virus-induced fibroblast interferon, thus exhibiting some properties of type I interferon. In vitro only CN 6134, the biologically active strain, could induce small amounts of interferon in spleen macrophage cultures. Macrophages from CN 6134 or BCG-infected athymic nu/nu mice produced similar interferon titers as their controls. It is concluded that infection with certain immunomodulators can activate splenic macrophages via a predominantly T-cell independent mechanism. Interferon in turn may operate locally as a mediator of immunoregulation.
在静脉注射细小棒状杆菌（C. parvum）CN 6134株或卡介苗（BCG）后，巨噬细胞被确定为小鼠脾细胞培养物中产生干扰素的主要来源。另一株细小棒状杆菌CN 5888在体内缺乏网状内皮系统（RES）刺激活性和佐剂活性，静脉注射时无效。产生干扰素活性需要蛋白质合成，表达抗病毒状态也需要蛋白质合成。抗病毒活性对pH 2相对稳定，并被抗病毒诱导的成纤维细胞干扰素的抗血清中和，因此表现出I型干扰素的一些特性。在体外，只有具有生物活性的CN 6134株能在脾巨噬细胞培养物中诱导产生少量干扰素。来自感染CN 6134或BCG的无胸腺裸鼠（nu/nu）的巨噬细胞产生的干扰素滴度与其对照相似。结论是，感染某些免疫调节剂可通过主要不依赖T细胞的机制激活脾巨噬细胞。干扰素反过来可能在局部作为免疫调节介质发挥作用。
