Naito H, Ito H, Wakisaka M, Kambegawa A, Shimazaki J
Invest Urol. 1981 Mar;18(5):337-40.
A 3-carboxymethyloxime conjugate of R 1881 was conjugated with bovine serum albumin and reacted with fluorescein isothiocyanate. Tissue sections from patients with benign prostatic hypertrophy were incubated with this compound. Fluorescence was observed in the cytoplasm of gland cells, although both positive and negative cells were distributed evenly in some areas. Neither the stroma nor the nuclei of epithelial cells were fluorescent. Preincubation with a 20 fold molar excess of R 1881-carboxymethyloxime-bovine serum albumin, 1 microM of R 1881, or 1 microM of triamcinolone acetonide diminished fluorescence of the section. Bovine serum albumin conjugated with fluorescein isothiocyanate did not stain the section. Therefore, the fluorescence observed in the sections seems to be attributable to the high affinity binding of R 1881. The intensity and number of fluorescent cells and the R 1881 binding estimated by a biochemical method were well correlated.
R1881的3 - 羧甲基肟共轭物与牛血清白蛋白结合，并与异硫氰酸荧光素反应。将良性前列腺增生患者的组织切片与该化合物一起孵育。在腺细胞的细胞质中观察到荧光，尽管在某些区域阳性和阴性细胞分布均匀。基质和上皮细胞核均无荧光。用20倍摩尔过量的R1881 - 羧甲基肟 - 牛血清白蛋白、1微摩尔的R1881或1微摩尔的曲安奈德预孵育可减弱切片的荧光。与异硫氰酸荧光素结合的牛血清白蛋白未对切片染色。因此，切片中观察到的荧光似乎归因于R1881的高亲和力结合。荧光细胞的强度和数量与通过生化方法估计的R1881结合情况具有良好的相关性。
