Ring J, Allen D H, Mathison D A, Spiegelberg H L
J Clin Lab Immunol. 1980 Mar;3(2):85-91.
Sixteen atopic patients with anaphylaxis to food, eczema, asthma and/or rhinitis were investigated for in vitro reactivity of their leukocytes and platelets to various stimuli. Leukocytes from two patients with anaphylaxis to foods had very high spontaneous release of histamine. Compared to non-atopic volunteers without respiratory disease, leukocytes from the other atopic patients showed increased histamine release after stimulation with methacholine in concentrations between 10(-2) and 10(-6) M. Histamine release induced by anti-IgE or anti-kappa chain serum was slightly decreased in atopics compared to controls, and was significantly decreased at low concentrations of anti-IgE (p less than 0.05). There was no significant difference of means for histamine release induced by the calcium ionophore A-23815. The uptake of 3H serotonin from platelet-rich plasma of atopic patients appeared to occur more slowly than in non-atopics. Serotonin release from washed platelets after stimulation with aggregated IgG was significantly lower in the atopic group (p less than 0.01). There was no significant difference in serotonin release induced by thrombin, epinephrine, ionophore or methacholine. Alterations in releasability of mediator containing cells to immunologic and non-immunologic stimuli may play a role in the expression of atopic disease.
对16名患有食物过敏、湿疹、哮喘和/或鼻炎的特应性患者的白细胞和血小板对各种刺激的体外反应性进行了研究。两名对食物过敏的患者的白细胞组胺自发释放量非常高。与无呼吸道疾病的非特应性志愿者相比，其他特应性患者的白细胞在浓度为10（-2）至10（-6）M的乙酰甲胆碱刺激后组胺释放增加。与对照组相比，特应性患者中抗IgE或抗κ链血清诱导的组胺释放略有降低，且在低浓度抗IgE时显著降低（p<0.05）。钙离子载体A-23815诱导的组胺释放平均值无显著差异。特应性患者富含血小板血浆中3H血清素的摄取似乎比非特应性患者发生得更慢。在特应性组中，聚集IgG刺激后洗涤血小板的血清素释放显著降低（p<0.01）。凝血酶、肾上腺素、离子载体或乙酰甲胆碱诱导的血清素释放无显著差异。含介质细胞对免疫和非免疫刺激的释放能力改变可能在特应性疾病的表达中起作用。
