Ullu E, Lunadei M, Fantoni A
Biochem Exp Biol. 1977;13(2):141-5.
Mouse satellite DNA sequences isolated by centrifugation in CS2SO4--Ag+ gradients are analyzed for buoyant density by CSCl density gradients and for their content of fast reassociating sequences by denaturation and partial reassociation. Our data suggest that in CS2SO4 gradients silver ions separate a satellite band which contains both fast reassociating G+C rich sequences and slow reassociating, A+T rich DNA sequences.
通过在Cs2SO4 - Ag+梯度中离心分离得到的小鼠卫星DNA序列，利用CsCl密度梯度分析其浮力密度，并通过变性和部分复性分析其快速复性序列的含量。我们的数据表明，在Cs2SO4梯度中，银离子分离出一个卫星带，该卫星带既包含快速复性的富含G+C的序列，也包含缓慢复性的富含A+T的DNA序列。
