通过银离子——硫酸铯密度梯度法分离得到的小鼠卫星DNA含有富含G+C、重退火缓慢的序列。
Mouse satellite DNA isolated by Ag+ -- CsSO4 density gradients contains G+C rich, slow reassociating sequences.
作者信息
Ullu E, Lunadei M, Fantoni A
出版信息
Biochem Exp Biol. 1977;13(2):141-5.
PMID:616298
Abstract
Mouse satellite DNA sequences isolated by centrifugation in CS2SO4--Ag+ gradients are analyzed for buoyant density by CSCl density gradients and for their content of fast reassociating sequences by denaturation and partial reassociation. Our data suggest that in CS2SO4 gradients silver ions separate a satellite band which contains both fast reassociating G+C rich sequences and slow reassociating, A+T rich DNA sequences.
摘要
通过在Cs2SO4 - Ag+梯度中离心分离得到的小鼠卫星DNA序列，利用CsCl密度梯度分析其浮力密度，并通过变性和部分复性分析其快速复性序列的含量。我们的数据表明，在Cs2SO4梯度中，银离子分离出一个卫星带，该卫星带既包含快速复性的富含G+C的序列，也包含缓慢复性的富含A+T的DNA序列。
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