Macaya G, Cortadas J, Bernardi G
Eur J Biochem. 1978 Mar;84(1):179-88. doi: 10.1111/j.1432-1033.1978.tb12155.x.
The dG+dC-rich fractions obtained by density gradient centrifugation of bovine DNA in Cs2SO4/BAMD [J. Cortadas, G. Macaya & G. Bernardi (1977) Eur. J. Biochem. 76, 13--19] were centrifuged in Cs2SO4/Ag+ density gradients. These experiments led to the preparation of the DNA components which had been detected (by analytical centrifugation in CsCl) in the Cs2SO4/BAMD fractions, and also of DNA components which had identical behaviors in Cs2SO4/BAMD gradients and identical buoyant densities in CsCl. A total of eight satellite components and 11 minor components, accounting for 23% and 4% of the bovine genome, respectively, were thus isolated and charcterized in their relative amounts and buoyant densities. The implications of these results on the interpretation of renaturation kinetic data on the bovine genome are discussed.
通过在Cs2SO4/BAMD中对牛DNA进行密度梯度离心获得的富含dG+dC的组分[J. 科尔塔达斯、G. 马卡亚和G. 贝尔纳迪（1977年）《欧洲生物化学杂志》76卷，第13 - 19页]在Cs2SO4/Ag+密度梯度中进行离心。这些实验导致制备出了在Cs2SO4/BAMD组分中已被检测到（通过在CsCl中进行分析离心）的DNA组分，以及在Cs2SO4/BAMD梯度中具有相同行为且在CsCl中具有相同浮力密度的DNA组分。总共分离出了八个卫星组分和11个次要组分，它们分别占牛基因组的23%和4%，并对其相对含量和浮力密度进行了表征。讨论了这些结果对牛基因组复性动力学数据解释的影响。
