Dutt M K
Microsc Acta. 1981 Jan;84(1):87-90.
This communication presents observations on the ability of an aqueous solution of cresyl violet to demonstrate DNA in deparaffinised rat tissue sections from which RNA has been extracted selectively with 90% phosphoric acid at 5 degrees C for 40 min. An aqueous solution of cresyl violet was found to be highly unstable and, therefore should be used when freshly prepared. The peak of maximum absorption of stained nuclei has been found to be at 560 nm. Staining of the nuclei following extraction of RNA has been due to the phosphate groups of DNA which is found to be in a native state.
本通讯介绍了关于甲酚紫水溶液在显示从经脱蜡处理的大鼠组织切片中提取DNA能力的观察结果，这些组织切片已在5℃下用90%磷酸选择性提取RNA 40分钟。发现甲酚紫水溶液极不稳定，因此应在新鲜制备时使用。已发现染色细胞核的最大吸收峰在560nm处。RNA提取后细胞核的染色归因于处于天然状态的DNA的磷酸基团。
