通过直接进样到液相色谱柱上简单快速地测定尿中5-羟吲哚-3-乙酸
Simple and rapid determination of 5-hydroxyindole-3-acetic acid in urine by direct injection on a liquid chromatographic column.
作者信息
Wahlund K G, Edlén B
出版信息
Clin Chim Acta. 1981 Feb 19;110(1):71-6. doi: 10.1016/0009-8981(81)90302-8.
5-Hydroxyindole-3-acetic acid has been determined at normal and increased levels in urine. The urine samples were filtered or centrifuged and then injected into a liquid chromatograph containing a reversed phase column with tributyl phosphate as stationary liquid phase and an aqueous buffer + methanol as eluent. 5-Hydroxyindole-3-acetic acid is detected in the eluate by a fluorometric detector coupled to the outlet of the separation column and the quantitation is performed by measurement of the peak heights of the sample and external standards. The percentage recovery was 97 and the precision 0.01 (sr). The method is suitable for the determination of increased levels of 5-hydroxyindole-3-acetic acid in urine from patients suffering from carcinoid tumours. Indole-3-acetic acid can be determined simultaneously.
已对尿液中正常水平和升高水平的5-羟基吲哚-3-乙酸进行了测定。将尿液样本过滤或离心，然后注入配备反相柱的液相色谱仪中，该反相柱以磷酸三丁酯为固定液相，以水性缓冲液+甲醇为洗脱液。通过与分离柱出口相连的荧光检测器在洗脱液中检测5-羟基吲哚-3-乙酸，并通过测量样品和外标物的峰高进行定量。回收率为97%，精密度为0.01（sr）。该方法适用于测定类癌肿瘤患者尿液中升高水平的5-羟基吲哚-3-乙酸。吲哚-3-乙酸可同时进行测定。
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