Miura T, Matsuda Y, Tsuji A, Katunuma N
J Biochem. 1981 Jan;89(1):217-22. doi: 10.1093/oxfordjournals.jbchem.a133184.
Antisera to purified gamma-glutamyltranspeptidase (gamma GTP) from human and rat kidney were prepared, and their reactivities toward purified gamma GTP from kidney, liver, and bile were tested. The following results were obtained: 1. On double immunodiffusion, Triton-solubilized gamma GTP, and papain-solubilized gamma GTP from rat kidney gave single precipitin lines which fused completely against antiserum to the purified enzyme from rat kidney. 2. An antigen-antibody complex of human kidney gamma GTP retained about 50% of the catalytic activity of the antigen. 3. Double immunodiffusion showed that the enzymes from human liver, kidney, and bile were immunologically identical. 4. Antiserum to rat kidney gamma GTP partially cross reacted with human gamma GTP, but antiserum to human gamma GTP reacted only very weakly with rat gamma GTP. It is concluded that gamma GTP of human liver, kidney, and bile are immunologically identical and that rat gamma GTP and human gamma GTP have certain antigenic determinants in common.
制备了针对人及大鼠肾脏纯化γ-谷氨酰转肽酶（γGTP）的抗血清，并检测了它们对肾脏、肝脏和胆汁中纯化γGTP的反应性。得到以下结果：1. 在双向免疫扩散中，大鼠肾脏经 Triton 溶解的γGTP 和经木瓜蛋白酶溶解的γGTP 产生单一沉淀线，与针对大鼠肾脏纯化酶的抗血清完全融合。2. 人肾脏γGTP 的抗原-抗体复合物保留了约 50%的抗原催化活性。3. 双向免疫扩散表明，人肝脏、肾脏和胆汁中的酶在免疫上是相同的。4. 针对大鼠肾脏γGTP 的抗血清与人γGTP 部分交叉反应，但针对人γGTP 的抗血清与大鼠γGTP 仅发生非常微弱的反应。得出的结论是，人肝脏、肾脏和胆汁中的γGTP 在免疫上是相同的，并且大鼠γGTP 和人γGTP 有某些共同的抗原决定簇。
