Kaplan G P, Hartman B K, Creveling C R
Neurochem Res. 1980 Aug;5(8):869-77. doi: 10.1007/BF00965786.
An antiserum to rat liver catechol-O-methyltransferase (COMT) was utilized in the immunological characterization of COMT from rat kidney, brain, and choroid plexuses, in addition to rat liver. The presence of anti-COMT activity was confirmed by the direct inhibition of the activity of the enzyme from rat liver by small quantities of the antiserum and by the inhibition of the activity of the enzyme from rat brain. The specificity of the antiserum was demonstrated both by immunoelectrophoresis of rat liver COMT, and by a partial purification of rat liver COMT in which changes in COMT specific activity were correlated with the appearance of a precipitin line in double-immunodiffusion experiments. The antigenic similarity of the enzyme derived from rat liver, kidney, brain, and choroid plexuses was demonstrated by the formation of a precipitin line of identity when preparations from these four tissues were diffused against the antiserum.
除大鼠肝脏外，还利用大鼠肝脏儿茶酚-O-甲基转移酶（COMT）抗血清对大鼠肾脏、大脑和脉络丛中的COMT进行免疫特性分析。通过少量抗血清直接抑制大鼠肝脏中该酶的活性以及抑制大鼠大脑中该酶的活性，证实了抗COMT活性的存在。通过大鼠肝脏COMT的免疫电泳以及大鼠肝脏COMT的部分纯化（其中COMT比活性的变化与双向免疫扩散实验中沉淀线的出现相关），证明了抗血清的特异性。当将来自这四个组织的制剂与抗血清进行扩散时，形成了一条同一性沉淀线，证明了源自大鼠肝脏、肾脏、大脑和脉络丛的酶的抗原相似性。
