高效液相色谱法测定尿中5-羟吲哚乙酸：一种全自动方法。

Determination of 5-hydroxyindoleacetic acid in urine by high-performance liquid chromatography: a fully automated method.

作者信息

Garnier J P, Bousquet B, Dreux C

出版信息

J Chromatogr. 1981 Jan 16;204:225-30. doi: 10.1016/s0021-9673(00)81663-5.

Abstract

High-performance liquid anion-exchange chromatography is combined with continuous flow spectrofluorometry at an optimized pH for the determination of 5-hydroxyindoleacetic acid (5-HIAA) in urine. Its chief advantages are speed (7 min per determination), enabling measurement of large series of samples, precision (coefficient of variation = 0.72%), sensitivity (detection limit: 1 pmol) and specificity (no interference with 5-HIAA precursors or with the drugs tested). Reference values (n = 112) are 25 +/- 25 mumol per 24 h. Comparison with a reference spectrofluorometric technique shows a correlation coefficient of 0.96.

摘要

高效液相阴离子交换色谱法与连续流动荧光光谱法相结合，在优化的pH条件下用于测定尿液中的5-羟吲哚乙酸（5-HIAA）。其主要优点包括速度快（每次测定7分钟），能够对大量样本进行测量，精密度高（变异系数=0.72%），灵敏度高（检测限：1皮摩尔）以及特异性强（不受5-HIAA前体或所测药物的干扰）。参考值（n = 112）为每24小时25±25微摩尔。与参考荧光光谱法技术比较，相关系数为0.96。