Cantey J R, Lushbaugh W B, Inman L R
J Infect Dis. 1981 Feb;143(2):219-30. doi: 10.1093/infdis/143.2.219.
RDEC-1 is a strain of Escherichia coli that, in rabbits, attaches to intestinal mucosal epithelial cells bereft of microvillar borders and causes diarrhea by an unknown mechanism. The stages of attachment of RDEC-1 bacteria to mucosal epithelial cells were examined using high-voltage electron microscopy of thick (0.5-micrometers) sections of ileum and cecum of rabbits with diarrhea. The tissues were stained with ruthenium red or antisera to strain RDEC-1 OK antigens. Micrographs, including stereopairs, demonstrated several stages of bacterial attachment. Bacteria were attached to the glycocalyxes of epithelial cell microvilli and to pedestal-like extrusions of the surfaces of epithelial cells lacking microvilli. Structures consistent with bacterial pili were rarely visualized. Attachment to microvilli appeared to be a result of the interaction of polysaccharides of the microvillar glycocalyx and the K antigen of the bacterial capsule.
RDEC-1是一种大肠杆菌菌株，在兔子体内，它附着于无微绒毛边界的肠道黏膜上皮细胞，并通过未知机制导致腹泻。利用腹泻兔子回肠和盲肠厚（0.5微米）切片的高压电子显微镜检查了RDEC-1细菌附着于黏膜上皮细胞的各个阶段。组织用钌红或抗RDEC-1 OK抗原血清染色。显微照片，包括立体对照片，展示了细菌附着的几个阶段。细菌附着于上皮细胞微绒毛的糖萼以及缺乏微绒毛的上皮细胞表面的基座样突起。很少能看到与细菌菌毛一致的结构。附着于微绒毛似乎是微绒毛糖萼的多糖与细菌荚膜K抗原相互作用的结果。
