将低倍立体显微镜改装用于扫描用异硫氰酸荧光素标记的细胞集落。

Adaptation of a low-power stereomicroscope to scan cell colonies labelled with fluorescein isothiocyanate.

作者信息

McMahon J B, Iype P T

出版信息

J Microsc. 1981 Feb;121(Pt 2):225-7. doi: 10.1111/j.1365-2818.1981.tb01216.x.

DOI:10.1111/j.1365-2818.1981.tb01216.x

Abstract

A stereomicroscope was modified for use in visualizing fluorescence-labelled cell-colonies at low magnifications. A xenon lamp and an FITC filter (Zeiss) were used to obtain exciting radiation to illuminate fluorescent-labelled specimens. The system is precise enough to distinguish between normal and cancer cell-colonies after specific interaction with FITC-conjugated antiserum.

摘要

对一台体视显微镜进行了改装，以便在低放大倍数下观察荧光标记的细胞集落。使用氙灯和FITC滤光片（蔡司）获得激发辐射，以照亮荧光标记的标本。该系统在与FITC偶联抗血清进行特异性相互作用后，精确到足以区分正常细胞集落和癌细胞集落。